You may be confused by my explanation in the Enemy Shooting tutorial. Let me explain perhaps more visually. You will need a single-pixel trigger sprite. that is, create a tileset that has 1x1 tiles, or maybe 3x3 and fill in the center pixel, if you want to make it a little easier. This sprite should be the trigger. If you are using larger tiles, i.e. 32x32 graphics and only filling in one color, then problems can arise like you've seen if you don't quite know how transparency works for sprites.
Just create an insignificantly small sprite.